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  • Title: Production and purification of the thermophilic bacteriophage TP-84.
    Author: Epstein I, Campbell LL.
    Journal: Appl Microbiol; 1975 Feb; 29(2):219-23. PubMed ID: 234714.
    Abstract:
    A new procedure for production and purification of the thermophilic bacteriophage TP-84 in high yields is described. Cultures of Bacillus stearothermophilus strain 10, enriched with nutrients to obtain heavy growth and to prevent sporulation and maintained at a pH of 6.5, were infected with the phage in a 100-liter fermentor. Addition of magnesium chloride (0.01 M) and a temperature of 58-C were essential for maximal phage production. Phage (5 times 1011 infective particles/ml) was precipitated with polyethylene glycol (molecular weight 6,000) in the presence of sodium chloride and was further purified by cesium chloride density centrifugation.
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