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Title: Size-dependent optical properties of bio-compatible ZnS:Mn nanocrystals and their application in the immobilisation of trypsin. Author: Sajimol Augustine M, Manzur Ali PP, Sapna K, Elyas KK, Jayalekshmi S. Journal: Spectrochim Acta A Mol Biomol Spectrosc; 2013 May; 108():223-8. PubMed ID: 23474481. Abstract: Chitosan capped zinc sulphide nanocrystals doped with manganese (ZnS:Mn) have been synthesised by chemical capping co-precipitation method and structurally characterised by XRD, TEM and EDXS techniques. The dependence of optical properties on the size of these bio-compatible ZnS:Mn nanocrystals was investigated by UV/Vis and photoluminescence (PL) spectroscopic techniques in aqueous solvents. A variation in molar concentration of the precursor, sodium sulphide, from 0.125 to 0.01 mol L(-1) is accompanied by a decrease in particle size. The excitonic peak in the UV/Vis spectra is found to be blue shifted with a decrease in size of the nanocrystals due to confinement effects. In the present study, trypsin was immobilised onto ZnS:Mn nanocrystals using glutaraldehyde (GA) as cross-linker, which was confirmed by photoluminescence (PL) and Fourier transform infrared (FTIR) spectroscopic studies. Results indicate that the activity of trypsin, immobilised onto chitosan modified nanocrystals, has improved upon cross-linking, which suggests that the immobilised trypsin has become more stable and active. This work highlights the prospects of potential applications of immobilised trypsin in therapeutic and diagnostic fields.[Abstract] [Full Text] [Related] [New Search]