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  • Title: [Application of flow cytometry in the detection of mouse malaria parasites in living cells].
    Author: Liu Y, Xi W.
    Journal: Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi; 2012 Oct 30; 30(5):340-2. PubMed ID: 23484270.
    Abstract:
    Peripheral blood samples were obtained from Plasmodium berghei-infected mice, and stained with Vybrant DyeCycle Green and anti-mouse CD71 PE. With normal mouse as negative control, the blood samples were tested by flow cytometry at 0, 30, and 60 min after staining. There was no positive signal in the erythrocytes from negative control and the unstained erythrocytes from the infected mouse. However, the positive signal was detected in the stained erythrocytes from the infected mouse. The results showed an adequate consistency by intra-class correlation coefficient (ICC) test (ICC = 0.999, P < 0.01). Flow cytometry with the use of Vybrant DyeCycle Green and anti-mouse CD71 PE in staining living cells can be used for the detection of malaria parasite-infected blood. In a given period, there is no negative impact on the detection of unfixed cells.
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