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  • Title: [Effect of gambogic acid on proliferation of SKM-1 cells and its mechanism].
    Author: Sun YY, Wang SJ, Chen BA, Xia GH, Ding JH, Gao C, Song HH, Guo QL, Zhang HW, Li CR, Zhou JF.
    Journal: Zhongguo Shi Yan Xue Ye Xue Za Zhi; 2013 Feb; 21(1):105-9. PubMed ID: 23484701.
    Abstract:
    The aim of this study was to explore the effect of gambogic acid (GA) on MDS SKM-1 cell proliferation, apoptosis and their possible mechanism. Cell proliferation was determined by MTT method. The apoptosis percentage and cell cycle regulation of SKM-1 cells were analyzed by flow cytometry. Morphological features were observed by light microscopy. The mRNA expression of bcl-2 and bax were detected by RT-PCR. The results showed that GA could inhibit the proliferation of SKM-1 cells in a dose- and time-dependent manner (IC50 was 0.37 µg/ml at 48 h), increase the apoptotic percentage of SKM-1 cells, and arrest cell cycle at the G0/G1. The expression of bax mRNA was up-regulated while that of bcl-2 mRNA was down-regulated in SKM-1 cells treated with GA for 48 h. It is concluded that GA can induce apoptosis, which may be related to its effect of arresting cells at phase of G0/G1 and down-regulating bcl-2/bax ratio.
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