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  • Title: A universal flow cytometry assay for screening carbohydrate-active enzymes using glycan microspheres.
    Author: Chandrasekaran A, Deng K, Koh CY, Takasuka T, Bergeman LF, Fox BG, Adams PD, Singh AK.
    Journal: Chem Commun (Camb); 2013 Jun 18; 49(48):5441-3. PubMed ID: 23536035.
    Abstract:
    We describe a simple, multiplexed assay that integrates glycan synthesis, bioconjugation to microspheres, fluorescent chemical/biochemical detection and multiparameter flow cytometric analysis to screen activities of different families of carbohydrate-active enzymes.
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