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  • Title: Steady state fluorescence polarization and Fourier transform infrared spectroscopy studies on membranes of functionally senescent human erythrocytes.
    Author: Fiorini R, Curatola G, Kantar A, Giorgi PL, Bertoli E, Tanfani F.
    Journal: Biochem Int; 1990; 20(4):715-24. PubMed ID: 2353921.
    Abstract:
    Human erythrocytes were separated using Percoll/Albumin density gradient centrifugation into 5 subpopulations which represent cell subpopulations of different ages. The physico-chemical properties of the membranes and their lipid extracts, prepared from the 5 subpopulations, were studied by 1,6-diphenyl-1,3,5-hexatriene fluorescence polarization and by Fourier Transform Infrared Spectroscopy respectively. The fluorescence polarization data show a decrease of membrane fluidity in the youngest erythrocyte fraction and the infrared spectroscopy reveals a lower degree of unsaturation of the lipids from this fraction. Changes of membrane fluidity among the 5 erythrocyte subpopulations is assigned to the variations of the degree of unsaturations of lipids rather than to variations of cholesterol content.
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