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  • Title: Bioimaging application of highly luminescent silica-coated ZnO-nanoparticle quantum dots with biotin.
    Author: Matsuyama K, Ihsan N, Irie K, Mishima K, Okuyama T, Muto H.
    Journal: J Colloid Interface Sci; 2013 Jun 01; 399():19-25. PubMed ID: 23541696.
    Abstract:
    We synthesized ZnO-nanoparticle quantum dots (QDs) as a fluorescent probe for biological applications. Highly luminescent silica-coated ZnO-nanoparticle QDs dispersed in an aqueous medium were synthesized using the sol-gel process. The ZnO-nanoparticle QDs were coated with silica to improve the water stability of the ZnO nanoparticles. NH2 groups were introduced on the surface of the silica-coated ZnO-nanoparticle QDs first by the addition of 3-aminopropyltrimethoxysilane and then by biotinylation with sulfosuccinimidyl-6-(biotin-amido) hexanoate (sulfo-NHS-LC-bioton). We demonstrated that avidin-immobilized agarose beads were tagged by the silica-coated ZnO-nanoparticle QDs with biotin by the selective avidin-biotin interaction, furnishing a fluorescent image upon excitation with UV light. Furthermore, use of the silica-coated ZnO-nanoparticle QDs with biotin in cell-labeling applications was attempted, and attachment of the silica-coated ZnO-nanoparticle QDs with biotin to nerve cells and actin filaments was achieved.
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