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  • Title: Efficient gene transfection in the neurotypic cells by star-shaped polymer consisting of β-cyclodextrin core and poly(amidoamine) dendron arms.
    Author: Liang B, Deng JJ, Yuan F, Yang N, Li W, Yin JR, Pu SX, Xie LC, Gao C, Zhang LM.
    Journal: Carbohydr Polym; 2013 Apr 15; 94(1):185-92. PubMed ID: 23544527.
    Abstract:
    In order to develop the effective vectors that had high gene transfection capability and low cytotoxicity in the neuronal cells, we tested the star-shaped polymer consisting of β-cyclodextrin core and poly(amidoamine) (PAMAM) dendron arms [β-CD-(D3)7] as the vector to transfect the human neuroblastoma SH-SY5Y cells. The physicochemical properties of the β-CD-(D3)7/plasmid DNA (pDNA) complexes were characterized by using gel electrophoresis, dynamic light scattering, transmission electron microscopy and zeta-potential experiments. Among the human neuroblastoma SH-SY5Y cells, β-CD-(D3)7/pDNA complex demonstrated a lower toxicity compared to those of PAMAM (G=4, with an ethylenediamine core)/pDNA complex. When the N/P ratio was over 20, it was observed that PAMAM had a faster increment in toxicity compared to β-CD-(D3)7. Fluorescent image, confocal microscopy image and flow cytometry showed that β-CD-(D3)7/pDNA complexes had significantly higher transgene activity than that of PAMAM/pDNA complexes. For example, the transfection efficiency was 20% and 7.5% for β-CD-(D3)7/pDNA and PAMAM/pDNA complexes, respectively. These results indicated that β-CD-(D3)7 might be a promising candidate for neurotypic cells gene delivery with the characteristics of good biocompatibility, relatively high gene transfection capability and potential in vivo gene delivery ability.
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