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  • Title: A real-time colorimetric and ratiometric fluorescent probe for sulfite.
    Author: Wu MY, He T, Li K, Wu MB, Huang Z, Yu XQ.
    Journal: Analyst; 2013 May 21; 138(10):3018-25. PubMed ID: 23563108.
    Abstract:
    A real-time colorimetric and ratiometric fluorescent probe based on modulating the intramolecular charge transfer (ICT) of the coumarin platform for selective detection of sulfite is presented. This reaction based probe utilized the Michael addition to the dicyano-vinyl group with the detection limit of 5.8 × 10(-5) M. The probe displayed a high selectivity for sulfite over other anions and reactive sulfur especially for biothiols including cysteine (Cys), homocysteine (Hcy) and glutathione (GSH), with about 100 nm blue shift and more than 230 times intensity ratios change of the emission spectrum. Meanwhile, it could be easily observed that the probe for sulfite changes from red to pale yellow by the naked eye, and from red to blue under UV lamp immediately after the sulfite is added. To the best of our knowledge, it is the fastest response probe for sulfite ever reported, which could give a colorimetric and ratiometric fluorescent response instantly.
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