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  • Title: Resonance light scattering determination of uranyl based on labeled DNAzyme-gold nanoparticle system.
    Author: Zhou B, Shi LF, Wang YS, Yang HX, Xue JH, Liu L, Wang YS, Yin JC, Wang JC.
    Journal: Spectrochim Acta A Mol Biomol Spectrosc; 2013 Jun; 110():419-24. PubMed ID: 23583878.
    Abstract:
    A resonance light scattering (RLS) method has been developed using a uranyl (UO2(2+)) specific DNAzyme and gold nanoparticles (AuNPs). In this strategy, the cleavage of the substrate strand (SDNA) of DNAzyme results in releasing a shorter duplex in the presence of UO2(2+), leading to the aggregation of AuNPs and the increase of RLS intensity. The response signals linearly correlated with the concentration of UO2(2+) over the range of 1.36×10(-8)-1.50×10(-7) mol L(-1). The limit of detection (LOD) is 4.09×10(-9) mol L(-1). The method has excellent selectivity and higher sensitivity. It could provide a promising potential for the detection of metal ions, and be benefit to extend the application of RLS method.
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