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  • Title: One-pot three-enzyme synthesis of UDP-Glc, UDP-Gal, and their derivatives.
    Author: Zou Y, Xue M, Wang W, Cai L, Chen L, Liu J, Wang PG, Shen J, Chen M.
    Journal: Carbohydr Res; 2013 May 24; 373():76-81. PubMed ID: 23584237.
    Abstract:
    A UTP-glucose-1-phosphate uridylyltransferase (SpGalU) and a galactokinase (SpGalK) were cloned from Streptococcus pneumoniae TIGR4 and were successfully used to synthesize UDP-galactose (UDP-Gal), UDP-glucose (UDP-Glc), and their derivatives in an efficient one-pot reaction system. The reaction conditions for the one-pot multi-enzyme synthesis were optimized and nine UDP-Glc/Gal derivatives were synthesized. Using this system, six unnatural UDP-Gal derivatives, including UDP-2-deoxy-Galactose and UDP-GalN3 which were not accepted by other approach, can be synthesized efficiently in a one pot fashion. More interestingly, this is the first time it has been reported that UDP-Glc can be synthesized in a simpler one-pot three-enzyme synthesis reaction system.
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