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  • Title: A multicentre study comparing two methods for serum free light chain analysis.
    Author: Lock RJ, Saleem R, Roberts EG, Wallage MJ, Pesce TJ, Rowbottom A, Cooper SJ, McEvoy ED, Taylor JL, Basu S.
    Journal: Ann Clin Biochem; 2013 May; 50(Pt 3):255-61. PubMed ID: 23605135.
    Abstract:
    BACKGROUND: Serum free light chain analysis is now well established in the investigation of monoclonal gammopathies. In the UK there has, until recently, been a single supplier of kits for such analysis. Recently, a second method using monoclonal antisera was introduced. We have compared the performance of these two kits in four routine laboratories. METHOD: Samples submitted for routine analysis (327 samples, 258 [79%] from patients with B-cell lymphoproliferative disease) for serum free light chains were tested by both technologies (Freelite, Binding Site and N Latex FLC, Siemens), according to the manufacturers' instructions. RESULTS: Qualitative data were available by both methods on 313 samples for serum free kappa chains and 324 samples for lambda free light chains. We found poor correspondence of 81% for kappa and 74% for lambda. Five percent of samples were significantly discordant in these assays. CONCLUSIONS: These assays perform very differently in clinical practice. They cannot be used interchangeably, especially if monitoring patient responses to therapy.
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