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  • Title: G3M T typing by dot immunobinding with monoclonal anti-G3M T antibody.
    Author: Fukuda M, Kishida T, Tamaki Y.
    Journal: Forensic Sci Int; 1990 Apr; 45(3):197-200. PubMed ID: 2361642.
    Abstract:
    Dilutions of 100 serum samples of various GM phenotypes were dotted onto a nitrocellulose membrane. The serum dot-blots were detected with peroxidase-labeled anti-G3M T monoclonal antibody (anti-G3M T MAb). Up to a 1 : 256 dilution could be G3M T-typed correctly. By use of anti-G3M T MAb and peroxidase-labeled anti-mouse IgG or the biotin-avidin system instead of use of labeled anti-G3M T MAb, up to a 1 : 512 or 1 : 1024, respectively, dilution was typable. As with previous work with G3M G MAb, the dot immunobinding (DIB) method for G3M T typing was found very simple and practical.
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