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  • Title: Gender-related difference in bloodstain RNA ratio stored under uncontrolled room conditions for 28 days.
    Author: Qi B, Kong L, Lu Y.
    Journal: J Forensic Leg Med; 2013 May; 20(4):321-5. PubMed ID: 23622483.
    Abstract:
    Bloodstain age is a parameter that can be used in crime scene investigations. Bloodstain age can be determined by measuring the 18S rRNA:β-actin mRNA ratio by Reverse Transcription-quantitative PCR (RT-qPCR). Since this ratio is a function of time, it can be used as an estimator of bloodstain age. However, it is important to validate the technique in a variety of scenarios before it can be applied. We investigated 18S rRNA:β-actin mRNA ratio in bloodstains from sixteen Chinese subjects in 28 days under uncontrolled room conditions. The ratio changed in a linear fashion. It was also found that the subjects' gender affected the relationship between time and the RNA ratio.
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