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  • Title: Screen-printed biosensor based on the inhibition of the acetylcholinesterase activity for the determination of codeine.
    Author: Asturias-Arribas L, Alonso-Lomillo MA, Domínguez-Renedo O, Arcos-Martínez MJ.
    Journal: Talanta; 2013 Jul 15; 111():8-12. PubMed ID: 23622519.
    Abstract:
    The current paper presents the chronoamperometric determination of codeine using screen-printed carbon electrodes that incorporate tetrathiafulvalene in the matrix of the working electrode, as mediator, and cross-linked acetylcholinesterase. Applying a potential of +250 mV, a 1mM solution of acetylthiocholine in electrolyte solution pH 7 gives an oxidation signal due to the dimerization of its metabolite after the reaction with the enzyme. This electrochemical signal is decreased by consecutive additions of a solution of codeine, which allows the performance of curves of calibration for the validation of this electrochemical method, giving a reproducibility of 3.31% (n=6) and a capability of detection of 20 µM. This type of inhibition has been studied by means of a Lineweaver-Burk plot. Additionally, the developed biosensor was used for the determination of the quantity of codeine in pharmaceutical commercial tablets and urine samples.
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