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  • Title: [Development and evaluation of a quantitative double antibodies sandwich ELISA assay for rIFN-alpha1b].
    Author: Wu MY, Ai YP, Cao Y, Wu S, Niu XX, Cheng YQ.
    Journal: Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi; 2012 Dec; 26(6):489-91. PubMed ID: 23627039.
    Abstract:
    OBJECTIVE: To develop a double antibody sandwich ELISA assay for quantitative determination of recombinant human interferon alpha1b. METHODS: Mouse monoclonal antibodies with different binding site on rIFN-alpha1b were screened to select optimized candidates as coating and HRP-labeled index antibodies respectively. And a double antibodies sandwich ELISA was assembled; the reliable lower detection limit, specificity, accuracy and reproducibility were evaluated and validated. RESULTS: The quantitative sandwich ELISA had a reliable lower detection limit of 10 ng/ml, with a liner detection range 10-100 ng/ml (R2 = 0.992), variation coefficient inter-plates is less than 10%. CONCLUSION: The developed sandwich ELISA was a sensitive and specific, accuracy and reproducibility method for quantitative determination of recombinant human interferon alpha1b in final product.
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