These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Aptamer-based sensing for thrombin in red region via fluorescence resonant energy transfer between NaYF₄:Yb,Er upconversion nanoparticles and gold nanorods.
    Author: Chen H, Yuan F, Wang S, Xu J, Zhang Y, Wang L.
    Journal: Biosens Bioelectron; 2013 Oct 15; 48():19-25. PubMed ID: 23639344.
    Abstract:
    In this work, we design a FRET system for sensitive and selective determination of thrombin in red region, in which NaYF₄:Yb,Er upconversion nanoparticles (UCNPs) act as donor and gold nanorods (Au NRs) act as acceptor. NaYF₄:Yb,Er UCNPs with a strong emission at 661 nm were successfully synthesized by tuning the doped ions ratio. Carboxyl-functionalized NaYF₄:Yb,Er UCNPs and Au NRs were then prepared and conjugated with the thrombin aptamers, respectively. The fluorescence emission band of NaYF₄:Yb,Er UCNPs (λ(max)=661 nm) highly overlaps with the absorption band of Au NRs(λ(max)=666 nm), which benefits from the large tunability of the spectrum band of Au NRs. A FRET system was then formed when thrombin was added to the mixture of NaYF₄:Yb,Er UCNPs and Au NRs, which were both modified thrombin aptamers. The fluorescence quenching efficiency of NaYF₄:Yb,Er UCNPs was increased in a thrombin concentration-dependent manner, which built the principle of thrombin quantification. The linear range was 2.5-90 nM in an aqueous buffer, and 3.75-112.5 nM in spiked human serum samples for thrombin. It also demonstrates a high selectivity to other biological species due to the specific binding. The measurement of thrombin in human plasma is satisfying, suggesting that the FRET system is of practical value in a complex biological sample matrix in red region.
    [Abstract] [Full Text] [Related] [New Search]