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  • Title: [Effect of homocysteine on gluconeogenesis in mice].
    Author: Wang Y, Yang L, Wang W, Feng W, Gui L, Wang F, Li S.
    Journal: Nan Fang Yi Ke Da Xue Xue Bao; 2013 Apr; 33(4):507-10. PubMed ID: 23644108.
    Abstract:
    OBJECTIVE: To investigate the expressions of glucose-6-phosphatase (G6Pase) and phosphoenolpyruvate carboxykinase (PEPCK) in the liver of mice with hyperhomocysteinemia (HHcy) and explore the mechanism of gluconeogenesis induced by homocysteine. METHODS: Fifty mice were randomly divided into normal control group (n=25) and HHcy group (n=25) and fed with normal food and food supplemented with 1.5% methionine, respectively. After 3 months of feeding, the fasting blood glucose and insulin levels were determined, and HOMA insulin resistance index (HOMA-IR) was calculated. The expressions of G6Pase and PEPCK in the liver of mice were detected using RT-PCR and Western blotting. RESULTS: The fasting blood glucose and insulin levels and HOMA-IR were significantly higher in HHcy group than in the control group (P<0.05). RT-PCR and Western blotting showed that the hepatic expressions of G6Pase and PEPCK mRNA and proteins increased significantly in HHcy group compared with those in the control group (P<0.05). CONCLUSION: Homocysteine promotes gluconeogenesis to enhance glucose output and contribute to the occurrence of insulin resistance.
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