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  • Title: Transport of tri- and dicarboxylic acids across the intestinal brush border membrane of calves.
    Author: Wolffram S, Bisang B, Grenacher B, Scharrer E.
    Journal: J Nutr; 1990 Jul; 120(7):767-74. PubMed ID: 2366111.
    Abstract:
    Transport of tri- and dicarboxylic acids across the intestinal brush border membrane was investigated using citrate and fumarate as transport substrates. The experiments were performed with brush border membrane vesicles isolated from calf proximal jejunum. Citrate and fumarate uptake by the brush border membrane vesicles occurred by a common Na(+)-dependent transport mechanism that appears to be specific for tri- and dicarboxylates. The protonated forms of citrate (citrate-(1) and citrate-(2] seem to be much better transported than the trivalent form, as indicated by the strong stimulation of citrate uptake at an extravesicular pH of 5.6 compared to that at pH 7.8. Furthermore, citrate transport across the intestinal brush border membrane appears to be mediated by an electroneutral process.
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