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  • Title: [Physicochemical properties and antiproliferative activity of recombinant Yersinia pseudotuberculosis L-asparaginase].
    Author: Pokrovskiĭ VS, Pokrovskaia MV, Aleksandrova SS, Andrianov RM, Zhdanov DD, Omel'ianiuk NM, Treshchalina EM, Sokolov NN.
    Journal: Prikl Biokhim Mikrobiol; 2013; 49(1):24-8. PubMed ID: 23662446.
    Abstract:
    The physicochemical, catalytic, and antiproliferative activity of a recombinant L-asparaginase from Yersinia pseudotuberculosis (YpA) have been studied. The following results were obtained: the K(M) value for L-asparagine is 17 +/- 0.9 microM, the optimal temperature is 60 degrees C, pH is 8.0, pI is 5.4 +/- 0.3, the L-glutaminase activity is no more than 5-6% of the L-asparaginase activity, and the antiproliferative activity on the Fisher L5178y lymphadenosis cell line comprised T/C = 136% (p < 0.001) at a 15% recovery rate. The described characteristic allows one to regard YpA as an antitumor enzyme with biological features similar to the L-asparaginase of E. coli.
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