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  • Title: Purification of nodule-specific uricase from soybean by arginine-sepharose affinity chromatography.
    Author: Larsen K.
    Journal: Prep Biochem; 1990; 20(1):1-9. PubMed ID: 2367346.
    Abstract:
    A method to purify uricase from soybean root nodules is described. The separation uses a single affinity chromatography step on Arginine-Sepharose, which was constructed by coupling L-Arginine to Activated CH-Sepharose 4B. Crude extracts were loaded onto small columns of Arginine-Sepharose and a significant retardation of uricase was observed. With a re-run of the fraction containing maximal uricase activity on the same column highly purified enzyme was obtained. Analysis by SDS-polyacrylamide gel electrophoresis revealed two protein bands. Analytical isoelectric focusing showed two isoforms of uricase, one dominating with pI 9.0 and a minor with pI 7.0.
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