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  • Title: Production, purification and some properties of cholesterol oxidase from a Streptomyces Sp.
    Author: Lartillot S, Kedziora P.
    Journal: Prep Biochem; 1990; 20(1):51-62. PubMed ID: 2367348.
    Abstract:
    A Streptomyces Sp strain producing appreciable levels of cholesterol oxidase was selected. In the culture medium, cholesterol oxidase activity can reach 13,000 UI/l. Cholesterol oxidase was purified by means of centrifugation, tangential ultrafiltration, ion-exchange chromatography and gel filtration with a yield of 42% to a specific activity of 54 UI/mg. Its molecular weight determined by SDS-PAGE was 55,000. Its optimum activity pH is about 7.
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