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  • Title: [Isolation of Ca 2+-activated photoprotein obelin from Obelia longissima and its use for the recording the outflow of Ca 2+ from fragmented sarcoplasmic reticulum of skeletal muscles].
    Author: Gitel'zon GI, Tugaĭ VA, Zakharchenko AN.
    Journal: Ukr Biokhim Zh (1978); 1990; 62(2):69-76. PubMed ID: 2368187.
    Abstract:
    A high-active stable preparation of obelin has been obtained from the luminescent hydroid Obelia longissima. The preparation is appropriate for determining free Ca2+ in the physiological range of its concentrations Obelin is shown possible to be used to record the processes of Ca2+ release from vesicles of the sarcoplasmic reticulum. In this case a rapid initial phase of Ca2+ outflux replaced by a slower one has been registered. A sharp increase of luminescence caused by the appearance of free Ca2+ in the medium has been registered under the effect of agents either increasing permeability of sarcoplasmic reticulum membranes for Ca2+ (A23187) or destroying the membrane (ethanol, triton X-100). The observed effects are confirmed, a radioactive label being used.
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