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  • Title: Simplified serum neutralization test based on enhanced green fluorescent protein-tagged classical swine fever virus.
    Author: Li Y, Shen L, Sun Y, Yuan J, Huang J, Li C, Li S, Luo Y, Qiu HJ.
    Journal: J Clin Microbiol; 2013 Aug; 51(8):2710-2. PubMed ID: 23698523.
    Abstract:
    The neutralization immunofluorescence test (NIFT), currently used for detecting neutralizing antibodies (NAbs) against classical swine fever virus (CSFV), is time-consuming. Here, a simplified neutralization test based on enhanced green fluorescent protein (EGFP)-tagged CSFV (EGFP-NT) was developed for direct detection of anti-CSFV NAbs without immunostaining. The relative sensitivity and specificity between EGFP-NT and NIFT or blocking enzyme-linked immunosorbent assay (ELISA) were both 100%. The NAb titers by EGFP-NT and the blocking rates by blocking ELISA showed a good correlation.
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