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  • Title: Solubilization of valine-sensitive acetohydroxy acid synthetase from Neurospora mitochondria.
    Author: Kuwana H, Date M.
    Journal: J Biochem; 1975 Jan 01; 77(1?):257-9. PubMed ID: 237000.
    Abstract:
    Acetohydroxy acid synthetase [EC 4.1.3.18] of Neurospora crassa has been solubilized from a mitochondrial pellet fraction by sonic treatment in 1.0 M potassium phosphate buffer at pH 7.5 containing 10 mM MgSO4 and centrifugation at 180,000 X g for 20 min. The soluble enzyme thus obtained has a high specific activity and a high sensitivity to valine comparable to the original mitochondrial pellet suspension when the activity was determined in high concentrations of potassium phosphate.
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