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  • Title: [Proteins in sperm and seminal plasma associated with human sperm resistance to cryopreservation].
    Author: Zhang XZ, Xiong CL.
    Journal: Zhonghua Nan Ke Xue; 2013 Mar; 19(3):214-7. PubMed ID: 23700725.
    Abstract:
    OBJECTIVE: To identify the proteins that could improve the resistance of human sperm to cryopreservation using comparative proteomics. METHODS: A total of 31 semen samples from 10 donors were divided into a high recovery and a low recovery group. Differentially expressed proteins in sperm and seminal plasma were detected and compared between the two groups by two-dimensional differential gel electrophoresis and mass spectrometry. RESULTS: Totally, 22 differentially expressed proteins were found in the two groups, 12 seminal plasma proteins, 9 sperm proteins, and 1 belonging to both. These identified proteins were involved in the maturation, movement, energy metabolism, DNA repair and other activities of spermatozoa. CONCLUSION: Many proteins were identified in sperm and seminal plasma that might influence the resistance of human sperm to cryopreservation.
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