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  • Title: Triplex PCR assay for the rapid identification of 3 major Vibrio species, Vibrio cholerae, Vibrio parahaemolyticus, and Vibrio fluvialis.
    Author: Vinothkumar K, Bhardwaj AK, Ramamurthy T, Niyogi SK.
    Journal: Diagn Microbiol Infect Dis; 2013 Aug; 76(4):526-8. PubMed ID: 23706502.
    Abstract:
    A triplex PCR assay was developed for the identification of 3 major Vibrio spp., Vibrio cholerae, Vibrio parahaemolyticus, and Vibrio fluvialis by targeting their haemolysin, haem-utilizing, and central regulatory genes, respectively. This simple, rapid, sensitive, and specific assay using cell lysates from 227 samples established its usefulness in research and epidemiology.
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