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  • Title: Photobleaching lifetimes of cyanine fluorophores used for single-molecule Förster resonance energy transfer in the presence of various photoprotection systems.
    Author: Cooper D, Uhm H, Tauzin LJ, Poddar N, Landes CF.
    Journal: Chembiochem; 2013 Jun 17; 14(9):1075-80. PubMed ID: 23733413.
    Abstract:
    Lengthening smFRET lifetimes: We investigated various photoprotection system combinations to find the combination that optimally extended the photobleach lifetime of a Cy3/Cy5 smFRET pair attached to a DNA hairpin in a single-molecule environment. We found that the glucose/glucose oxygen-scavenging solution in combination with redox-based photostabilization solutions yielded the longest average photobleaching lifetimes.
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