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  • Title: A highly sensitive G-quadruplex-based luminescent switch-on probe for the detection of polymerase 3'-5' proofreading activity.
    Author: Leung KH, He HZ, Zhong HJ, Lu L, Chan DS, Ma DL, Leung CH.
    Journal: Methods; 2013 Dec 15; 64(3):224-8. PubMed ID: 23748144.
    Abstract:
    We report herein a luminescent switch-on label-free G-quadruplex-based assay for the rapid and sensitive detection of polymerase proofreading activity using a novel iridium(III) complex as a G-quadruplex-selective probe. The interaction of the iridium(III) complex with the G-quadruplex motif facilitates the highly sensitive switch-on detection of polymerase proofreading activity. Using T4 DNA polymerase (T4 pol) as a model enzyme, the assay achieved high sensitivity and selectivity for T4 pol over other tested enzymes.
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