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  • Title: Liquid chromatography and fluorescence detection of vitamin A in animal feeds, finished feeds, and premixes.
    Author: Thorpe VA.
    Journal: J Assoc Off Anal Chem; 1990; 73(3):463-6. PubMed ID: 2376553.
    Abstract:
    A simple liquid chromatographic method for vitamin A (retinol) in animal feeds is described. The feed is saponified, diluted to minimize interferences, and extracted into petroleum ether with a single step. The analysis is sensitive and specific with liquid chromatography and a fluorescence detector. The minimum level of detection is 15 ng/mL, which is equivalent to 10,000 units/lb vitamin A. The method includes a stable and reproducible standardization of vitamin A that is used to calibrate standard peak heights in terms of units retinol/mL. Guarantees of 10,000 units/lb up to premix levels can be analyzed with good recoveries and precision.
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