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  • Title: A method for determination of UDP-GlcNAc: GlcNAcβ1-6(GlcNAcβ1-2)Manα1-R [GlcNAc to Man] β1-4N-acetylglucosaminyltransferase VI activity.
    Author: Taguchi T, Taniguchi N.
    Journal: Methods Mol Biol; 2013; 1022():299-305. PubMed ID: 23765670.
    Abstract:
    To characterize and purify glycosyltransferases, it is essential to establish a simple and sensitive assay method. Here, we describe a method for determination of the activity of GnT VI (UDP-GlcNAc: GlcNAcβ1-6(GlcNAcβ1-2)Manα1-R [GlcNAc to Man] β1-4N-acetylglucosaminyltransferase VI) using a fluorescently labeled oligosaccharide.
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