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  • Title: Use of PCR to estimate the prevalence of Equus caballus papillomavirus in aural plaques in horses.
    Author: Gorino AC, Oliveira-Filho JP, Taniwaki SA, Basso RM, Zakia LS, Araujo JP, Borges AS.
    Journal: Vet J; 2013 Sep; 197(3):903-4. PubMed ID: 23773810.
    Abstract:
    Aural plaques occur on the skin of the medial surface of the pinnae of horses. In this study the presence of Equus caballus papillomavirus (EcPV)-3 and -4 DNA was assessed in 45 such plaques using a 'touchdown' PCR. Papillomaviruses (PVs) were detected in 62.3% (28/45) of samples: EcPV-3 and -4 DNA in 8.89% (4/45) and 37.78% (17/45) of samples, respectively, with 15.56% (7/45) of samples exhibiting co-infection. Viral DNA was not detected in 37.78% (17/45) of samples, suggesting the possible existence of other equine PVs. Neither EcPV-3 nor -4 were detected in negative control skin. This study is the first to evaluate the prevalence of these two viruses in equine aural plaques.
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