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  • Title: [Effects of silver ion of silver nanoparticles on gap junctional intercellular communication of human skin cells].
    Author: Qin Y, Deng FR, Wei HY, Han LM, Xu JH, Guo XB.
    Journal: Beijing Da Xue Xue Bao Yi Xue Ban; 2013 Jun 18; 45(3):412-6. PubMed ID: 23774919.
    Abstract:
    OBJECTIVE: To further investigate the effects of silver ion (Ag+) dissolved from silver nanoparticles (AgNps) on gap junctional intercellular communication (GJIC) between HaCaT cells. METHODS: In this study, 20 000×g of 1 g/L AgNps suspension were centrifuged at 4 °C for 2 h, then the supernatant was collected as Ag(+) stock solution and measured by inductively coupled plasma mass spectrometry (ICP-MS); GJIC was detected by the scrape loading/dye transfer assay; connexin 43 (Cx43) protein and mRNA level were estimated by Western-blot and RT-PCR, respectively. RESULTS: Different concentrations of Ag(+) (0.01, 0.1 and 1.0 mg/L) did not affect the GJIC significantly. No notable changes were observed in expression of Cx43 protein and mRNA. CONCLUSION: The characteristics of Ag(+) and the effects on gap junctional intercellular communication between HaCaT cells may be different from those of AgNps.
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