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  • Title: Simultaneous analysis of eprosartan and hydrochlorothiazide in tablets by high-performance liquid chromatography.
    Author: Patel HU, Suhagia BN, Patel CN.
    Journal: Pharm Methods; 2011 Apr; 2(2):143-7. PubMed ID: 23781445.
    Abstract:
    OBJECTIVE: A simple, precise and accurate isocratic reversed phase (RP) column high-performance liquid chromatographic (HPLC) method has been developed for simultaneous analysis of eprosartan (EPR) and hydrochlorothiazide (HCT) in tablet formulations. MATERIALS AND METHODS: Isocratic RP-HPLC separation was achieved on phenomenex C18 column (250 × 4.6 mm i.d., 5 μm particle size) using mobile phase composed of 0.5% formic acid-methanol-acetonitrile [(80 : 25 : 20 v/v/v) pH, 2.80 ± 0.04] at a flow rate of 1.0 ml/min. The retention time for EPR and HCT was 7.69 ± 0.10 and 4.24 ± 0.09 minutes, respectively. The detection was performed at 272 nm. RESULTS: The method was linear in the concentration range of 60-600 μg/ml for EPR and 2.5-25 μg/ml for HCT with a correlation coefficient of 0.9992 and 0.9997, respectively. The repeatability for six samples was 0.53 and 0.61 % RSD for EPR and HCT, respectively. The accuracy (recovery) was found to be in the range of 99.46 to 100.61% for EPR and 99.06 to 100.93% for HCT, respectively. CONCLUSIONS: The method was validated and successfully used for determination of the drugs in tablets.
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