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  • Title: Radioprotective effect of adenine on irradiation-induced apoptosis.
    Author: Wang XY, Ma ZC, Shao S, Hong Q, Wang YG, Tan HL, Lu XQ, Dong Z, Gao Y.
    Journal: Chin J Nat Med; 2013 Mar; 11(2):139-44. PubMed ID: 23787180.
    Abstract:
    AIM: To investigate the radioprotective effect of adenine on irradiated lymphocytes and discover the possible mechanisms of protection. METHODS: Lymphocytes were pretreated for 12 h with adenine (0.001-0.1 μmol·L(-1)) and then exposed to 4 Gy radiation. Cell viability was observed by the MTS assay, apoptosis was detected by Annexin V-FITC/PI, DNA ladder, and caspase 3/7 activity. Caspase-9, Bax, and Bcl-2 gene expression was investigated by RT-PCR. RESULTS: Irradiation increased cell death and DNA fragmentation. Pretreatment with adenine significantly reversed this tendency. Furthermore, several anti-apoptotic characteristics of adenine were determined, including the ability to inhibit caspase 3/7, upregulate B-cell lymphoma (Bcl-2) and downregulate Bcl-2- associated X (Bax), capase-9 gene expression in 4 Gy-irradiated AHH-1 cells. CONCLUSION: The results suggest that adenine had a radioprotective effect to inhibit apoptosis in a concentration dependent manner.
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