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  • Title: Rapid assay for detecting gyrA and parC mutations associated with fluoroquinolone resistance in Enterobacteriaceae.
    Author: Nakano R, Okamoto R, Nakano A, Nagano N, Abe M, Tansho-Nagakawa S, Ubagai T, Kikuchi-Ueda T, Koshio O, Kikuchi H, Ono Y.
    Journal: J Microbiol Methods; 2013 Sep; 94(3):213-6. PubMed ID: 23816531.
    Abstract:
    We developed a PCR-RFLP assay to detect mutations in the quinolone-resistance determining regions of gyrA and parC associated with fluoroquinolone resistance in Enterobacteriaceae. The assay detected mutations associated with reduced susceptibility to fluoroquinolones and may therefore serve as a specific, rapid, inexpensive, and simple testing alternative.
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