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  • Title: Development of a real-time SYBR Green PCR assay for the rapid detection of Dermatophilus congolensis.
    Author: García A, Martínez R, Benitez-Medina JM, Risco D, Garcia WL, Rey J, Alonso JM, Hermoso de Mendoza J.
    Journal: J Vet Sci; 2013; 14(4):491-4. PubMed ID: 23820221.
    Abstract:
    Methods such as real time (RT)-PCR have not been developed for the rapid detection and diagnosis of Dermatophilus (D.) congolensis infection. In the present study, a D. congolensis-specific SYBR Green RT-PCR assay was evaluated. The detection limit of the RT-PCR assay was 1 pg of DNA per PCR reaction. No cross-reaction with nucleic acids extracted from Pseudomonas aeruginosa, Mycobacterium tuberculosis, Staphylococcus aureus, or Austwickia chelonae was observed. Finally, the RT-PCR assay was used to evaluate clinical samples collected from naturally infected animals with D. congolensis. The results showed that this assay is a fast and reliable method for diagnosing dermatophilosis.
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