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  • Title: Protein-binding aptamer assisted signal amplification for the detection of influenza A (H1N1) DNA sequences based on quantum dot fluorescence polarization analysis.
    Author: Zhang J, Tian J, He Y, Chen S, Jiang Y, Zhao Y, Zhao S.
    Journal: Analyst; 2013 Sep 07; 138(17):4722-7. PubMed ID: 23826611.
    Abstract:
    We report a fluorescence polarization platform for H1N1 detection based on the construction of a DNA functional QD fluorescence polarization probe and a bi-functional protein binding aptamer (Apt-DNA). The assay has a linear range from 10 nM to 100 nM with a detection limit of 3.45 nM and is selective over the mismatched bases.
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