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  • Title: Lipase entrapment in PVA/Chitosan biodegradable film for reactor coatings.
    Author: Batista KA, Lopes FM, Yamashita F, Fernandes KF.
    Journal: Mater Sci Eng C Mater Biol Appl; 2013 Apr 01; 33(3):1696-701. PubMed ID: 23827626.
    Abstract:
    This study reports the development and characterization of novel biodegradable film, based on chitosan and polyvinyl alcohol containing lipase entrapped. The films showed a thickness of 70.4 and 79 μm to PVA/Chitosan and PVA/Chitosan/Lipase, respectively. The entrapment of lipase in PVA/Chitosan film resulted in increasing of 69.4% tensile strength (TS), and 52.4% of elongation. SEM images showed the formation of a continuous film, without pores or cracks. The lipase entrapment efficiency was estimated in 92% and the films were repeatedly used for 25 hydrolytic cycles, maintaining 62% of initial activity. The PVA/Chitosan/Lipase film was used for olive oil hydrolysis of high performance. These results indicate that PVA/Chitosan/Lipase is a promising material for biotechnology applications such as triacylglycerol hydrolysis and biodiesel production.
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