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  • Title: Peptide mapping of the four subunits of the mouse DNA polymerase alpha-primase complex.
    Author: Takada-Takayama R, Tada S, Hanaoka F, Ui M.
    Journal: Biochem Biophys Res Commun; 1990 Jul 31; 170(2):589-95. PubMed ID: 2383257.
    Abstract:
    We report a simple, two-step method (phosphocellulose and immunoaffinity column chromatographies) for purification of the mouse DNA polymerase alpha-primase complex. The advantages of this method over other procedures are its simplicity and rapidity, with little loss by proteolysis. Sedimentation analysis in a glycerol density gradient of the immunoaffinity-purified fraction revealed that four polypeptides with molecular weights of 180,000, 68,000, 54,000 and 46,000 in the enzyme fraction form a physical complex. Peptide mapping by reversed phase-high performance liquid chromatography demonstrated unequivocally that these four polypeptides constituting the complex are different entities.
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