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  • Title: Purification and characterisation of the placental-like alkaline phosphatase from ovarian epithelial tumours.
    Author: Koyama I, Hirano K, Makiya R, Stendahl U, Stigbrand T.
    Journal: Br J Cancer Suppl; 1990 Jul; 10():6-11. PubMed ID: 2383481.
    Abstract:
    The placental alkaline phosphatase was purified by immunoaffinity chromatography from ovarian epithelial tumours to homogeneity. Up to 40% of the catalytical phosphatase activity in these tumours was derived from this placental type alkaline phosphatase (PLAP). The purified enzyme were similar to those of PLAP, whereas the PLAP-like isozyme was more heat-stable and resistant to 8 M urea than PLAP. The amino terminal sequence of the PLAP-like enzyme demonstrated heterogeneity at position three in the N-terminal end compared with PLAP. Phenyl-Sepharose affinity chromatography and different lectin chromatographies demonstrated the tumour-derived enzyme to be microheterogeneous, both with regard to concanavalin A binding and hydrophobicity properties.
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