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  • Title: Determination of chidamide in rat plasma by LC-MS and its application to pharmacokinetics study.
    Author: Wang X, Chen M, Wen C, Zhang Q, Ma J.
    Journal: Biomed Chromatogr; 2013 Dec; 27(12):1801-6. PubMed ID: 23857175.
    Abstract:
    A sensitive and selective liquid chromatography mass spectrometry method for determination of chidamide in rat plasma was developed. After addition of linezolid as internal standard, protein precipitation by acetonitrile-methanol (9:1, v/v) was used as sample preparation. Chromatographic separation was achieved on a Zorbax SB-C18 (2.1 × 150 mm, 5 µm) column with acetonitrile-0.1% formic acid as mobile phase with gradient elution. An electrospray ionization source was applied and operated in positive ion mode; selective ion monitoring mode was used for quantification using target fragment ions m/z 391.5 for chidamide and m/z 338.5 for the IS. Calibration plots were linear over the range of 10-2000 ng/mL for chidamide in rat plasma. The lower limit of quantification for chidamide was 10 ng/mL. The mean recovery of chidamide in plasma was in the range of 86.6-92.1%. The coefficients of variation of intra-day and inter-day precision were both <12%. This method is simple and sensitive and was applied successfully in a pharmacokinetic study of chidamide to rats.
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