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  • Title: Prevention of arsenic-mediated reproductive toxicity in adult female rats by high protein diet.
    Author: Mondal S, Mukherjee S, Chaudhuri K, Kabir SN, Kumar Mukhopadhyay P.
    Journal: Pharm Biol; 2013 Nov; 51(11):1363-71. PubMed ID: 23859609.
    Abstract:
    CONTEXT: The detrimental effects of arsenic on female reproductive functions may involve overt oxidative stress. Casein and pea [Pisum sativum Linn. (Fabaceae)] proteins have antioxidant properties. OBJECTIVE: To investigate the role of casein- and pea-supplemented high-protein diet (HPD) in utero-ovarian protection from arsenic toxicity. MATERIALS AND METHODS: Adult female Wistar rats were orally gavaged with vehicle (Gr-I) or arsenic at 3 ppm/rat/d (Gr-II and Gr-III) for 30 consecutive days, when they were maintained on either regular diet containing 18% protein (Gr-I and Gr-II), or HPD containing 27% protein in the form of casein (20%) and pea (7%) (Gr-III). Reproductive functions were evaluated using a battery of biochemical and histological techniques. RESULTS: As compared to Gr-I, the Gr-II rats suffered from loss of estrous cyclicity, reduction in weight (mg/100 g body weight) of ovary (Gr-I: 54.3 ± 4.2 versus Gr-II: 35.8 ± 1.6; p < 0.001) and uterus (Gr-I: 161.7 ± 24.6 versus Gr-II: 94.44 ± 13.2; p < 0.05), utero-ovarian degeneration, attenuated ovarian activities (unit/mg tissue/h) of Δ(5), 3β-hydroxysteroid dehydrogenase (Gr-I: 3.41 ± 0.12 versus Gr-II: 2.31 ± 0.09; p < 0.01) and 17β-hydroxysteroid dehydrogenase (Gr-I: 3.82 ± 0.57 versus Gr-II: 1.24 ± 0.19; p < 0.001), and decreased serum estradiol level (pg/ml) (Gr-I: 61.5 ± 2.06 versus 34.1 ± 2.34; p < 0.001). Ovarian DNA damage was preponderant with blatant generation of malondialdehyde (nM/mg tissue; Gr-I: 15.10 ± 2.45 versus Gr-II: 29.51 ± 3.44; p < 0.01) and attenuated superoxide dismutase activity (unit/mg tissue) (Gr-I: 2.18 ± 0.19 versus Gr-II: 1.33 ± 0.18; p < 0.05). The Gr-III rats were significantly protected from these ill effects of arsenic. DISCUSSION AND CONCLUSION: HPD, by way of antioxidant properties, may find prospective role in the protection of reproductive damage caused by arsenic.
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