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  • Title: Inhibition of peroxisomal hydroxypyruvate reductase (HPR1) by tyrosine nitration.
    Author: Corpas FJ, Leterrier M, Begara-Morales JC, Valderrama R, Chaki M, López-Jaramillo J, Luque F, Palma JM, Padilla MN, Sánchez-Calvo B, Mata-Pérez C, Barroso JB.
    Journal: Biochim Biophys Acta; 2013 Nov; 1830(11):4981-9. PubMed ID: 23860243.
    Abstract:
    BACKGROUND: Protein tyrosine nitration is a post-translational modification (PTM) mediated by nitric oxide-derived molecules. Peroxisomes are oxidative organelles in which the presence of nitric oxide (NO) has been reported. METHODS: We studied peroxisomal nitroproteome of pea leaves by high-performance liquid chromatography with tandem mass spectrometry (LC-MS/MS) and proteomic approaches. RESULTS: Proteomic analysis of peroxisomes from pea leaves detected a total of four nitro-tyrosine immunopositive proteins by using an antibody against nitrotyrosine. One of these proteins was found to be the NADH-dependent hydroxypyruvate reductase (HPR). The in vitro nitration of peroxisomal samples caused a 65% inhibition of HPR activity. Analysis of recombinant peroxisomal NADH-dependent HPR1 activity from Arabidopsis in the presence of H2O2, NO, GSH and peroxynitrite showed that the ONOO(-) molecule caused the highest inhibition of activity (51% at 5mM SIN-1), with 5mM H2O2 having no inhibitory effect. Mass spectrometric analysis of the nitrated recombinant HPR1 enabled us to determine that, among the eleven tyrosine present in this enzyme, only Tyr-97, Tyr-108 and Tyr-198 were exclusively nitrated to 3-nitrotyrosine by peroxynitrite. Site-directed mutagenesis confirmed Tyr198 as the primary site of nitration responsible for the inhibition on the enzymatic activity by peroxynitrite. CONCLUSION: These findings suggest that peroxisomal HPR is a target of peroxynitrite which provokes a loss of function. GENERAL SIGNIFICANCE: This is the first report demonstrating the peroxisomal NADH-dependent HPR activity involved in the photorespiration pathway is regulated by tyrosine nitration, indicating that peroxisomal NO metabolism may contribute to the regulation of physiological processes under no-stress conditions.
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