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  • Title: Vaccinia virus expression and sequence of an avian influenza nucleoprotein gene: potential use in diagnosis.
    Author: Harley VR, Hudson PJ, Coupar BE, Selleck PW, Westbury H, Boyle DB.
    Journal: Arch Virol; 1990; 113(1-2):133-41. PubMed ID: 2386434.
    Abstract:
    The nucleoprotein (NP) gene from avian influenza strain A/Shearwater/Aust/1/72 (H6N5) was cloned, sequenced, and expressed in vaccinia virus for the production of potent sera in immunised rabbits. The NP gene is 1565 bp and shares greater than 95% amino acid sequence identity with other NPs of the avian subtype. The recombinant NP expressed by vaccinia virus comigrated with endogenous A/Shearwater/Aust/1/72 NP by Western blot analysis. Polyclonal rabbit sera raised against recombinant NP was evaluated in an antigen capture ELISA system as a potential diagnostic tool for the detection of avian influenza. All type A strains, comprising several HA and NA subtypes, but not type B nor other avian viruses, were detected.
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