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  • Title: VE-cadherin status as an indicator of microvascular permeability.
    Author: Monaghan-Benson E, Burridge K.
    Journal: Methods Mol Biol; 2013; 1046():335-42. PubMed ID: 23868598.
    Abstract:
    VE-cadherin phosphorylation and binding partner status are important indicators of endothelial permeability. Here we describe several techniques aimed at discerning total tyrosine phosphorylation levels of VE-cadherin, VE-cadherin phosphorylation on specific tyrosine residues, and the ability of VE-cadherin to bind its binding partner beta-catenin. Taken together, these approaches to studying VE-cadherin status on microvascular endothelial cells are excellent complements to traditional permeability assays.
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