These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Enhanced production of γ-cyclodextrin by optimization of reaction of γ-cyclodextrin glycosyltransferase as well as synchronous use of isoamylase.
    Author: Wang L, Wu D, Chen J, Wu J.
    Journal: Food Chem; 2013 Dec 01; 141(3):3072-6. PubMed ID: 23871061.
    Abstract:
    In this study, we examined the production of γ-cyclodextrin (γ-CD) using γ-cyclodextrin glycosyltransferase (γ-CGTase) from Bacillus clarkii 7364. We investigated a variety of factors that affect the yield of γ-CD, including key factors such as solvent, substrate, temperature, pH, enzyme dosage. After optimising the reaction parameters, a 50.4% (w/w) yield of γ-CD was obtained with 15% (w/v) potato starch and cyclododecanone (which could be easily removed). In order to further enhance the production of γ-CD, we constructed the γ-CGTase mutant A223K by site-directed mutagenesis, and used it synchronously with the isoamylase obtained from Thermobifida fusca. At pH 7.0 and 50 °C, 72.5% (w/w) γ-CD yield was achieved after 10h, which was 22.1% higher than that obtained using γ-CGTase alone, and represents the highest yield reported to date. The substantially higher yield and selectivity of γ-CD obtained in the present study provides the basis for the industrial-scale production of γ-CD.
    [Abstract] [Full Text] [Related] [New Search]