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  • Title: Aptamer-linked biosensor for thrombin based on AuNPs/thionine-graphene nanocomposite.
    Author: Zhang Z, Luo L, Zhu L, Ding Y, Deng D, Wang Z.
    Journal: Analyst; 2013 Sep 21; 138(18):5365-70. PubMed ID: 23877321.
    Abstract:
    A novel protocol of electrochemical thrombin aptasensor based on a goldnanoparticles/thionine-graphene (AuNPs/Th-G) nanocomposite modified glassy carbon electrode was presented. Graphene was non-covalently functionalized by thionine viaπ-stacking interaction, and then AuNPs were electrodeposited onto the Th-G surface. The morphology and conductivity of the AuNPs/Th-G nanocomposite were characterized by scanning electron microscopy, cyclic voltammetry and electrochemical impedance spectroscopy. The ethanethiol-substituted oligonucleotide probe was immobilized onto the surface of the AuNPs/Th-G nanocomposite to prepare an electrochemical biosensing platform. The aptamer-thrombin reaction was monitored by differential pulse voltammetry. Under optimum conditions, the proposed biosensor exhibits high sensitivity and a low detection limit for thrombin determination. The thrombin could be quantified in a wide range of 0.5 to 40 nM and with a low detection limit of 0.093 nM (S/N = 3).
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