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  • Title: Role for the mitochondrial inner membrane in the maturation of the precursor to ornithine carbamyl transferase.
    Author: Côté C, Poirier J, Boulet D.
    Journal: Biochem Biophys Res Commun; 1990 Aug 16; 170(3):1273-80. PubMed ID: 2390092.
    Abstract:
    The precursor to ornithine carbamyl transferase (pOCT) is cleaved at two N-terminal sites when imported into intact mitochondria but only at the N-proximal site when incubated with a membrane-free mitochondrial lysate or matrix fraction. Disruption of the mitochondrial membrane system by sonication, freeze-thaw, or lysis with non-ionic detergents blocks the processing of pOCT to its mature form. Mitoplasts prepared from protease-inactivated, import-incompetent mitochondria recover full processing activity; disruption of the inner membrane impairs the maturation process i.e. causes the loss of the mitoplasts' ability to transform pOCT into OCT. The data reveal a dependency of a maturation event on a "specific" interaction between a precursor protein and the mitochondrial inner membrane probably to position and/or to expose the correct N-distal cleavage site of the presequence.
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