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  • Title: Effect of reactive oxygen species and nitric oxide in the neural control of intrarenal haemodynamics in anaesthetized normotensive rats.
    Author: Ahmeda AF, Rae MG, Johns EJ.
    Journal: Acta Physiol (Oxf); 2013 Oct; 209(2):156-66. PubMed ID: 23910436.
    Abstract:
    AIMS: This study examined the interaction between reactive oxygen species and nitric oxide (NO) in mediating the decrease in renal blood flow (RBF) evoked by sympathetic renal nerve stimulation (RNS). METHODS: Groups of male Wistar rats were subjected to RNS at different frequencies prior to, and following, an infusion of: (i) tempol, the superoxide dismutase (SOD) mimetic, (ii) tempol plus the hydrogen peroxide-degrading enzyme catalase (tem + cat), (iii) diethyldithiocarbamic acid (DETC), a SOD inhibitor, (iv) the nitric oxide synthase (NOS) inhibitor, L-nitro-arginine methyl ester (L-NAME) alone, or (v) L-NAME followed by tempol, into the kidney cortico-medullary border (CMB). Blood perfusion within the cortical (CBP) and medullary (MBP) regions of the kidney was measured using Laser-Doppler flowmetry. RESULTS: Infusion of tempol CMB significantly attenuated RNS-evoked reductions in CBP (by 22% at 8 Hz; P < 0.05), but not MBP. When tempol and catalase were co-infused to reduce both ROS and hydrogen peroxide (H2 O2 ), respectively, there was a significantly greater attenuation of the RNS-evoked reduction in CBP compared with that of tempol alone. Infusion of either DETC or L-NAME alone did not significantly affect the CBP or MBP responses to RNS. Similarly, RNS following tempol infusion with L-NAME also had no effect on CBP and MBP over and above the group that received tempol alone. CONCLUSION: These results suggest that reactive oxygen species such as superoxide and H2 O2 have a direct role in reducing renal vascular compliance in response to RNS, rather than indirectly through scavenging NO.
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